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. Author manuscript; available in PMC: 2018 Jun 27.
Published in final edited form as: Adv Exp Med Biol. 2018;1029:101–107. doi: 10.1007/978-981-10-7545-2_9

Figure 2. FACS gating strategy for TVC progeny isolation.

Figure 2

Samples electroporated with blank buffer, Mesp>tagRFP, MyoD905>GFP and Hand-r>tagBFP fluorescent reporters are used to gate the TVC progeny populations. a. Gate P1 for single cell population based off the FS and SS from the blank sample. b. Gate the tagRFP+ cell population using the sample electroporated with Mesp>tagRFP. c. Gate the GFP+ cell population using the sample electroporated with MyoD905>GFP. d. Gate the tagBFP+ cell population using the sample electroporated with Hand-r>tagBFP. e. Define the cells only have tagRFP as P2 in the sample co-electroporated with Mesp>tagRFP, MyoD905>GFP and Hand-r>tagBFP. f. Analyze P2 with gates set for tagRFP and tagBFP, define the tagRFP-tagBFP+ cell population as P3. P3 is TVC progeny that we sort for downstream genome-wide analysis.