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. 2018 Jun 22;11(4):971–978. doi: 10.1016/j.tranon.2018.05.011

Figure 2.

Figure 2

Characterization of the IGF-II axis in Hep3B and HepG2 cells. (A) Cells were treated with vehicle or 20 μg/ml DX-2647 for 48 hours and then tested on activated RTK arrays. Black dots indicate a positive activation signal. (1) p-IR, (2) p-IGF-IR, and (3) p-HER3. Signals in each array corner are positive controls. (B) Western analyses of IGF-II species contained within concentrated and conditioned cell supernatants, treated with vehicle or an O-glycosyltransferase inhibitor (OGTi). IGF-II isoforms are indicated by black arrows. (C) Western analyses of IGFBP expression in cell lysates isolated from Hep3B and HepG2 cells. IGFBP2, IGFBP5, and IGFBP6 were not detected. (D) Immunoprecipitation analysis of bioavailable IGF-II bound from concentrated and conditioned cell supernatants followed by IGF-II blotting. IGF-II species are indicated by black arrows.