The distributions of the ITS sequences of the validated dataset ITS-V (left) and its extension with the ITS sequences of the “Top 50 Most Wanted Fungi” dataset (right). The five groups in green, red, blue and pink represent 3 810, 2 210, 2 064, 1 483, and 574 ITS sequences of Sordariomycetes, Eurotiomycetes, Dothideomycetes, Agaricomycetes, and Leotiomycetes, respectively. All 2 024 sequences of the “Top 50 Most Wanted Fungi” dataset are in chocolate colour. The group in turquoise colour contains 1 145 sequences that have no a class name given in the database. The 3D coordinates of the sequences were computed using fMLC (Vu et al. 2018). The sequences were visualized using the rgl package in R (https://r-forge.r-project.org/projects/rgl/).