Fig. 2.

Col2-Cre:Tsc1 fl/fl metatarsals have a growth advantage ex vivo. (A) Growth curves of metatarsals collected from three-day old mice and cultured for six days ex vivo. (B) Metatarsals were collected immediately before culture or after one day in culture (contralateral controls), then stained for pS6, Ki67 (immunohistochemistry) or COL10A1 (in situ hybridization). For Ki67 staining, the region directly above the hypertrophic zone is expanded (pink box). For COL10A1 staining, the yellow dashed line denotes the border between the cartilage and the primary spongiosa. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)