Determination of miR-20a expression in human MM cell lines and clinical samples via RT-qPCR. (A) RT-qPCR detection of miR-20a in plasma cells derived from bone marrow of healthy donors (normal) and MM patients. (B) Expression of miR-20a in the MM cell lines MM.1S, U266 and RPMI-8226 compared with nPCs. Each sample was analyzed in triplicate and error bars represent standard deviation. *P<0.05 vs. nPCs. nPCS, normal plasma cells; MM, multiple myeloma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; miR, microRNA.