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. 2018 Jun 25;13:98. doi: 10.1186/s13023-018-0843-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — DPSCs were induced to differentiate towards adipogenic, osteogenic, and chondrogenic lineages in a 2D culture. Phase-contrast microscopy. a-b: Oil Red O staining shows lipids deposits in red after 21 days of adipogenic differentiation. c-d: von Kossa staining identifies calcium deposits at the end of osteogenic protocol. e-f: Alcian blue staining shows the presence of GAGs. A, C, E: CT12. B, D, F: NF87