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. 2018 Jun 26;18:135. doi: 10.1186/s12870-018-1326-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Schematic representation of the hpt and GUS gene constructs used in the transformation experiments. P-Ubi, ubiquitin promoter (1.6 Kb); hpt, hygromycin resistance gene (1 Kb); GUS, gusA gene (1.8 Kb); 35S, 35S terminator (200 bp); Nos, Nos terminator (258 bp). About ~ 3 Kb hpt DNA cassette was used for stable transformation