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. 2018 Jun 26;18:135. doi: 10.1186/s12870-018-1326-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Biolistic wheat transformation process with hygromycin selection. a Freshly isolated immature embryo; (b) 2 week-old embryogenic callus before bombardment; (c) Shoot regeneration on 50 mg/L hygromycin selection medium; (d) Shoot and root regeneration on 50 mg/L hygromycin selection medium; (e) Plantlet recovery in 1/2 MS medium; (f) T₀ transgenic plants in the greenhouse