Table 2.
Plasmids used in this study
| Plasmid | Specific features | Source |
|---|---|---|
| Vectors | ||
| pGK425 | Yeast multi-copy type single-gene expression vector containing PGK1 promoter, PGK1 terminator, 2μ origin, and LEU2 marker | [37] |
| pAT425 | Yeast multi-copy type two-gene expression vector containing ADH1 and TDH3 promoters and terminators, 2μ origin, LEU2 marker | [39] |
| pATP422 | Yeast multi-copy type three-gene expression vector containing ADH1, TDH3 and PGK1 promoters and terminators, 2μ origin, ADE2 marker | [39] |
| Yeast acetolactate synthase (ALS) expressions | ||
| pGK425-ILV2 | pGK425, expression of Saccharomyces cerevisiae ALS (ILV2) gene | [4] |
| pGK425-ILV2c | pGK425, expression of truncated version of S. cerevisiae ALS lacking the N-terminal (mitochondrial) signal sequence (ILV2c) gene | [38] |
| ALS expressions | ||
| pGK425-ALSAo | pGK425, expression of Aspergillus oryzae ALS (ALS or AO090009000123) gene | This study |
| pGK425-alsSBs | pGK425, expression of Bacillus subtilis ALS (alsS) gene | This study |
| pGK425-alsCg | pGK425, expression of Corynebacterium glutamicum ALS (als or Cgl1271) gene | This study |
| pGK425-ilvBEc | pGK425, expression of Escherichia coli ALS (ilvB) gene | This study |
| pGK425-ilvGEc | pGK425, expression of E. coli ALS (ilvG) gene | This study |
| pGK425-ilvIEc | pGK425, expression of E. coli ALS (ilvI) gene | This study |
| pGK425-AHAS2Gm | pGK425, expression of Glycine max ALS (AHAS2) gene | This study |
| pGK425-AHAS3Gm | pGK425, expression of G. max ALS (AHAS3) gene | This study |
| pGK425-ilvGHe-1 | pGK425, expression of Halomonas elongata ALS (ilvG) gene 1 [HELO_1296] | This study |
| pGK425-ilvGHe-2 | pGK425, expression of H. elongata ALS (ilvG) gene 2 [HELO_2761] | This study |
| pGK425-alsLl | pGK425, expression of Lactococcus lactis ALS (als) gene | This study |
| pGK425-alsLp | pGK425, expression of Lactobacillus plantarum ALS (als) gene | This study |
| pGK425-alsSg-1 | pGK425, expression of Streptomyces griseus ALS (als) gene 1 [SGR_4789] | This study |
| pGK425-alsSg-2 | pGK425, expression of S. griseus ALS (als) gene 2 [SGR_6206] | This study |
| pGK425-alsTf | pGK425, expression of Thermobifida fusca ALS (als) gene [Tfu_0611] | This study |
| pGK425-ALS1Zm | pGK425, expression of Zea mays ALS (ALS1) gene [100285396] | This study |
| High-activity ALS expressions (codon optimized) | ||
| pGK425-ilvBEcOp | pGK425, expression of E. coli codon-optimized ilvB (ilvBEcOp) gene | This study |
| pGK425-alsLpOp | pGK425, expression of L. plantarum codon-optimized als (alsLpOp) gene | This study |
| pGK425-alsTfOp | pGK425, expression of T. fusca codon-optimized als (alsTfOp) gene | This study |
| pATP422-alsLpOp | pATP422, expression of alsLpOp gene by PGK1 promoter | This study |
| Acetolactate decarboxylase (ALDC) expressions (codon optimized) | ||
| pATP422-alsLpOp-aldcBsOp | pATP422, expressions of alsLpOp gene by PGK1 promoter and B. subtilis codon-optimized ALDC (aldcBsOp) gene by TDH3 promoter | This study |
| pATP422-alsLpOp-aldcEaOp | pATP422, expressions of alsLpOp gene by PGK1 promoter and Enterobacter aerogenes codon-optimized ALDC (aldcEaOp) gene by TDH3 promoter | This study |
| pATP422-alsLpOp-aldcKpOp | pATP422, expressions of alsLpOp gene by PGK1 promoter and Klebsiella pneumoniae codon-optimized ALDC (aldcKpOp) gene by TDH3 promoter | This study |
| pATP422-alsLpOp-aldcLlOp | pATP422, expressions of alsLpOp gene by PGK1 promoter and L. lactis codon-optimized ALDC (aldcLlOp) gene by TDH3 promoter | This study |
| Yeast butanediol dehydrogenase (BDH) expression | ||
| pATP422-alsLpOp-BDH1 | pATP422, expressions of alsLpOp gene by PGK1 promoter and S. cerevisiae BDH (BDH1) gene by ADH1 promoter | This study |
| pAT425-BDH1 | pAT425, expressions of S. cerevisiae BDH (BDH1) gene by ADH1 promoter | This study |