Table 3.
Yeast transformants used in this study
| Transformant | Host strain | Plasmid | Gene 1 (ALS) | Gene 2 (ALDC) | Gene 3 (BDH) |
|---|---|---|---|---|---|
| YIDB001 | YPH499 | pGK425 | – | – | – |
| YIDB002 | YPH499 | pGK425-ILV2 | ILV2 (S. cerevisiae) | – | – |
| YIDB003 | YPH499 | pGK425-ILV2c | ILV2c (S. cerevisiae) | – | – |
| YIDB004 | YPH499 | pGK425-ALSAo | ALSAo (A. oryzae) | – | – |
| YIDB005 | YPH499 | pGK425-alsSBs | alsSBs (B. subtilis) | – | – |
| YIDB006 | YPH499 | pGK425-alsCg | alsCg (C. glutamicum) | – | – |
| YIDB007 | YPH499 | pGK425-ilvBEc | ilvBEc (E. coli) | – | – |
| YIDB008 | YPH499 | pGK425-ilvGEc | ilvGEc (E. coli) | – | – |
| YIDB009 | YPH499 | pGK425-ilvIEc | ilvIEc (E. coli) | – | – |
| YIDB010 | YPH499 | pGK425-AHAS2Gm | AHAS2Gm (G. max) | – | – |
| YIDB011 | YPH499 | pGK425-AHAS3Gm | AHAS3 (G. max) | – | – |
| YIDB012 | YPH499 | pGK425-ilvGHe-1 | ilvGHe-1 (H. elongata) | – | – |
| YIDB013 | YPH499 | pGK425-ilvGHe-2 | ilvGHe-2 (H. elongata) | – | – |
| YIDB014 | YPH499 | pGK425-alsLl | alsLl (L. lactis) | – | – |
| YIDB015 | YPH499 | pGK425-alsLp | alsLp (L. plantarum) | – | – |
| YIDB016 | YPH499 | pGK425-alsSg-1 | alsSg-1 (S. griseus) | – | – |
| YIDB017 | YPH499 | pGK425-alsSg-2 | alsSg-2 (S. griseus) | – | – |
| YIDB018 | YPH499 | pGK425-alsTf | alsTf (T. fusca) | – | – |
| YIDB019 | YPH499 | pGK425-ALS1Zm | ALSZm (Z. mays) | – | – |
| YIDB022 | YPH499 | pGK425-ilvBEcOp | ilvBEcOp (E. coli, optimized) | – | – |
| YIDB023 | YPH499 | pGK425-alsLpOp | alsLpOp (L. plantarum, optimized) | – | – |
| YIDB024 | YPH499 | pGK425-alsTfOp | alsTfOp (T. fusca, optimized) | – | – |
| YIDB025 | YPH499 | pATP422-alsLpOp | alsLpOp (L. plantarum, optimized) | – | – |
| YIDB030 | YPH499 | pATP422-alsLpOp-aldcBsOp | alsLpOp (L. plantarum, optimized) | aldcBsOp (B. subtilis, optimized) | – |
| YIDB031 | YPH499 | pATP422-alsLpOp-aldcEaOp | alsLpOp (L. plantarum, optimized) | aldcEaOp (E. aerogenes, optimized) | – |
| YIDB032 | YPH499 | pATP422-alsLpOp-aldcKpOp | alsLpOp (L. plantarum, optimized) | aldcKpOp (K. pneumoniae, optimized) | – |
| YIDB033 | YPH499 | pATP422-alsLpOp-aldcLlOp | alsLpOp (L. plantarum, optimized) | aldcLlOp (L. lactis, optimized) | – |
| YIDB034 | YPH499 | pATP422-alsLpOp-BDH1 | alsLpOp (L. plantarum, optimized) | – | BDH1 (S. cerevisiae) |
| YHI010 | YSM046 | pGK425-alsLpOp | alsLpOp (L. plantarum, optimized) | – | – |
| YHI011 | YSM046 | pATP422-alsLpOp-aldcLlOp | alsLpOp (L. plantarum, optimized) | aldcLlOp (L. lactis, optimized) | – |
| YHI027 | YPH499 | pATP422-alsLpOp-aldcLlOp/pAT425-BDH1 | alsLpOp (L. plantarum, optimized) | aldcLlOp (L. lactis, optimized) | BDH1 (S. cerevisiae) |
| YHI030 | YSM046 | pATP422-alsLpOp-aldcLlOp/pAT425-BDH1 | alsLpOp (L. plantarum, optimized) | aldcLlOp (L. lactis, optimized) | BDH1 (S. cerevisiae) |
| YSHB001 | YSM021 | pATP422-alsLpOp-aldcLlOp/pAT425-BDH1 | alsLpOp (L. plantarum, optimized) | aldcLlOp (L. lactis, optimized) | BDH1 (S. cerevisiae) |