Fig. 4.

Cytokine release upon DC activation of antigen-specific T cells. The release of IL-2, IL-4, IL-10 and IFN-γ by OT-I and OT-II T cells following stimulation with different APC subsets was investigated. L-DC and cDC-like subsets were sorted from 21-day co-cultures established with Lin⁻ BM from Act-mOVA mice above 5G3 stroma. Control CD8⁺ cDC and CD8⁻ cDC subsets were sorted from Act-mOVA spleen. These four APC subsets, and a NIL control, were incubated with column purified CD8⁺ OT-I T cells and CD4⁺ OT-II T cells from spleen for 4 days. The supernatant of co-cultures was collected, tested for the presence of cytokines using an Elisa array kit (Qiagen), and production calculated as A₄₅₀ of sample relative to A₄₅₀ of a standard provided with the kit