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Study workflow. Three human colorectal cancer cell lines (HT-29, LoVo and LS174T) were selected as proxies of clinical cancers and cultured in vitro (left) or used to establish tumor xenografts (right). DNA and RNA were isolated from cells, supernatants and tumor xenografts. ctDNAs and miRNAs were isolated from blood plasma. dPCR, RT-ddPCR and RT-qPCR were performed to detect KRAS G12D and G13D, PIK3CA H1047R, FBXW7 R505C mutations and miR-141, miR-221 and miR-222 expression levels
