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. 2018 Jun 26;37:124. doi: 10.1186/s13046-018-0788-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — miRNA expression in cultured cells and supernatants. (a) 2D ddPCR plots of miR-222-3p in the three indicated cell lines (upper) and their culture supernatants (lower). All cDNAs from cells were diluted 1:100 for accurate Poisson distribution analysis. (b) miR-141-3p, miR-221-3p and miR-222-3p were quantitated in cells (dots) and supernatants (diamonds). All data are normalized for total mRNA content, and expressed as copy/μl of miRNA in the original samples. Standard deviation was calculated from three independent experiments