Table 1.
TaqMan assays (primer and probe sets) employed in dPCR, RT-ddPCR and RT-experiments
| Assay (primers and probe) | Assay codea | Employed technology |
|---|---|---|
| KRAS p. G12D | KRAS_521, ID AH6R5PIb | dPCR |
| KRAS p. G13D | KRAS_ 532, ID AHD2BW0b | dPCR |
| PIK3CA p. H1047R | PIK3CA_775, ID AHPAVCDb | dPCR |
| FBXW7 p. R505C | custom assay, ID ANZTGJUb | dPCR |
| hsa-miR-141-3p | 000463c | RT-ddPCR, RT-qPCR |
| hsa-miR-221-3p | 000524c | RT-ddPCR, RT-qPCR |
| hsa-miR-222-3p | 002276c | RT-ddPCR, RT-qPCR |
| cel-miR-39-3p | 000200c | RT-ddPCR, RT-qPCR |
aAll assays are provided from Thermo Fisher Scientific. bThis assay was used for dPCR on genomic DNA and ctDNA. cThis assay was used for reverse transcription and amplification of microRNAs in ddPCR and quantitative PCR