Table 1.
Induction of organized embryogenic structures (OES), friable embryogenic structures (FEC) from cassava cultivars and response to MeSPY1-VIGS cassava mosaic disease challenge
| Cultivar name | Resistance type | Organized embryogenic structures (OES) induction frequency (%) | Friable Embryo-genic Callus (FEC) induction (Yes/No) | Number of dead plants/total plants challenged with MeSPY1-VIGS | Resistance/ susceptibility to cassava mosaic disease | ||
|---|---|---|---|---|---|---|---|
| Wildtype | OES-derived | Wildtype | OES-derived | ||||
| NASE 3 | CMD1 | 24 | No | 1/8 | 3/8 | Resistant | Resistant |
| NASE 14 | CMD1 | 81 | Yes | 0/9 | 0/10 | Resistant | Resistant |
| TMS 30572 | CMD1 | 28 | No | 2/9 | 1/8 | Resistant | Resistant |
| TME 204 | CMD2 | 81 | Yes | 1/9 | 7/7a | Resistant | Susceptible |
| TME B7 | CMD2 | 95 | Yes | 1/9 | 6/6 | Resistant | Susceptible |
| TME 419 | CMD2 | 58 | Yes | 7/9 | 6/6 | Susceptible | Susceptible |
| TMS 96/1632 | CMD3 | 63 | No | 0/6 | 0/9 | Resistant | Resistant |
| TMS 98/0505 | CMD3 | 55 | Yes | 0/8 | 0/9 | Resistant | Resistant |
| TMS 98/0581 | CMD3 | 66 | No | 0/7 | 0/10 | Resistant | Resistant |
| TMS 92/0326 | Unknown | 89 | Yes | 1/8 | 1/7 | Resistant | Resistant |
| TMS 92/0057 | Unknown | 76 | No | 0/12 | 0/5 | Resistant | Resistant |
| TMS 95/0289 | Unknown | 3 | No | 3/11 | NA | Resistant | Not tested |
| TMS 98/2132 | Unknown | 79 | No | 0/6 | 0/4 | Resistant | Resistant |
| NR03/0155 | Unknown | 53 | No | 0/9 | 0/8 | Resistant | Resistant |
| TMS 91/02324 | Unknown | 53 | Yes | 0/10 | 0/9 | Resistant | Resistant |
| TMS 98/0002 | Unknown | 78 | No | 0/10 | 0/10 | Resistant | Resistant |
| TMS 01/0040 | Unknown | 59 | Yes | 0/10 | 0/9 | Resistant | Resistant |
| TMS 01/1206 | Unknown | 66 | Yes | 0/7 | 0/8 | Resistant | Resistant |
| TMS 01/1371 | Unknown | 64 | No | 0/8 | 0/9 | Resistant | Resistant |
| Mbundamali | Unknown | Not determined | Not tested | 10/10 | 5/5 | Susceptible | Susceptible |
| 60444 | Susceptible | 90 | Yes | 9/9 | NA | Susceptible | Not tested |
aFEC derived TME 204 used as control (FEC-TME 204)