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. 2018 Jun 26;11:179. doi: 10.1186/s13068-018-1169-x

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Hydrolysis of CC by CmChi1. The reactions contained 50 μg CmChi1 and 1% (w/v) CC, and were performed in sodium citrate buffer (pH 5.2) at 50 °C, and aliquots were withdrawn at different time intervals and analyzed by HPLC. Numbers 1–6 represent GlcNAc to (GlcNAc)₆. a HPLC profiles of reaction products from CC. b The time courses of products generated from CC. Products were quantified from the respective areas using standard curves of (GlcNAc)_1–6