Table 1.

Oligonucleotide primers used for PCR

Primer	Sequence (5ʹ-3ʹ)	Design basis	Position in Cmchi1 gene (5ʹ-3ʹ)
Cmchi1PF	GCTGCCACCCCGACACCGGTTTCGGCTACC	Peptide fragments 1	253–282
Cmchi1PR	TGCGTTCTTCAGCACCTTGTAGTCCTC	Peptide fragments 2	1744–1770
ARB1	GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT	Arbitrary primer for TAIL-PCR	None
ARB2	GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC	Arbitrary primer for TAIL-PCR	None
ARB3	GGCCACGCGTCGACTAGTAC	Arbitrary primer for TAIL-PCR	None
F-SP1	GGCGCCGCTCGCGGCACCTAC	Specific primer for TAIL-PCR	1711–1731
F-SP2	GGTCGTGGCTGGACTGGCGTGCCG	Specific primer for TAIL-PCR	1654–1677
R-SP1	GTCGTCGCCACCCCGACACCG	Specific primer for TAIL-PCR	292–312
R-SP2	CGGTGTCGGGGTGGCGACGAC	Specific primer for TAIL-PCR	344–366
Cmchi1-F	CATGCCATGGATGTCGCAAATCAATCGCTTC	Forward primer for CmChi1	1–21
Cmchi1-R	CCGCTCGAGTTACTTGTTCATGTTGCCCATG	Reverse primer for CmChi1	1950–1977