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Purification of recombinant CmChi1 by different methods
| Purification method | Total activity (U) | Total protein (mg) | Specific activity (U/mg)a | Purification (fold) | Recovery yield (%) |
|---|---|---|---|---|---|
| Crude enzyme | 198.6 | 521.1 | 0.4 | 0 | 100 |
| Ni–NTA resin | 139.6 | 12.3 | 11.4 | 29.9 | 70.3 |
| Chitinase–glycogen complex precipitation | 176.8 | 11.6 | 15.3 | 40.2 | 89.0 |
aEnzymatic activity was measured in 50 mM citrate buffer (pH 5.2) at 50 °C for 30 min using 1% (w/v) CC as the substrate
