Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 26;9(3):e00975-18. doi: 10.1128/mBio.00975-18

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.

PMC Copyright notice

FIG 3 — Expression of RARP-2 during infection. (A) FLAG-tagged SS-RARP-2, Iowa-RARP-2, MTase, and pRAMF2 empty vector control were overexpressed from R. rickettsii Iowa in Vero76 cells (MOI of 1) and sampled at 12, 24, 48, and 72 hpi for immunoblotting with an anti-FLAG antibody. Dots to the right of the bands indicate the major breakdown products of Iowa-RARP-2 at 48 and 72 hpi. Reduced signal at 72 h is presumably due to further proteolysis of the products. Tubulin was used as a loading control. (B) Cultures infected with SS-RARP-2-FLAG were fixed at times corresponding to panel A and stained with anti-FLAG antibodies (red) for observation by immunofluorescence assay. GFP-expressing rickettsiae are green. Bar, 10 µm. Corresponding Nomarski differential interference contrast images are provided.