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. 2018 Jun 26;9(3):e00975-18. doi: 10.1128/mBio.00975-18

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FIG 8 — Type IV secretion of RARP-2. (A) FLAG-tagged SS-RARP-2 expressed in R. rickettsii Iowa was coimmunoprecipitated using anti-RvhD4 antibody or preimmune serum (Pre). Input (In) and immunoprecipitated proteins (IP) were detected by Western blotting (WB) using anti-RvhD4 antibody (left) or anti-FLAG antibody (right). The expected size for SS-RvhD4 is 67 kDa (arrow), and the expected size for SS-RARP-2 is 70 kDa (arrow). (B) Endogenous R. typhi RARP-2 (Rt-RARP-2) homolog was coimmunoprecipitated using anti-RvhD4 antibody or preimmune serum (Pre). Input (In) and precipitated proteins (IP) were detected by Western blotting (WB) using anti-RvhD4 antibody or anti-Rt-RARP-2 antibody. The expected size for Rt-RvhD4 is 67 kDa (arrow), and the expected size for Rt-RARP-2 is 70 kDa (arrow). Bands near 50 kDa represent immunoglobulin heavy chain.