TABLE 4 .

TpCM-2^a

Component	Amt for 50 ml	Final concn	Manufacturer/catalog no.
1× CMRL 1066 medium without l-glutamine or phenol red	37 ml	0.8×	United States Biological/C5900-03A
Sodium pyruvate	364 µl	0.73 mM	Sigma/S8636
0.1% resazurin	50 µl	0.001%	Sigma/R7017
MOPS (1 M), pH 7.5	1 ml	20 mM	Sigma/M3183
NaHCO3 (7.5%)	1.08 ml	19.2 mM	Sigma/M8761
l-Glutamine (200 mM)	500 µl	2 mM	Sigma/G6152
100× d-glucose (15% in water)	500 µl	To 17.6 mM	Sigma/G6152
d-Mannitol (10 g/dl) (10% in water)	80 µl	0.88 mM	Sigma/M-1902
l-Histidine (5 g/dl) (5% in water)	80 µl	0.52 mM	Sigma/H6034
dl-Dithiothreitol	4 mg	0.52 mM	Sigma/D9779
Fetal bovine serum, heat inactivated	10 ml	20%	Sigma/F4135

^aTpCM-2, T. pallidum cultivation medium 2; MOPS, morpholinepropanesulfonic acid.