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. 2018 Jun 27;24:34. doi: 10.1186/s10020-018-0035-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Evaluation the effect of overexpression of AR and DHT treatment on expression of EGFR in VCaP cells. a Immunoblot analysis was performed to examine the expression of AR in VCaP cells that were transfected with pCMV control vector (pCMV-Ctrl) or pCMV-AR vector (pCMVAR) and followed by treatment with DHT or vehicle control. b Expression of EGFR protein in VCaP cells that were transfected with pCMV control vector (pCMV-Ctrl) or pCMV-AR vector (pCMVAR) and followed by treatment with DHT or vehicle control. Antibody against GAPDH was used as loading control. Data presented is average of three independent experiments (±SD). p < 0.05 is indicated by “*”, p ≤ 0.01 is indicated by “**”