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. 2018 Jun 27;24:34. doi: 10.1186/s10020-018-0035-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Evaluation of the effect of overexpression of AR in the presence or absence of DHT treatment on MMP-9 and EGFR expression in PCa cells. a Immunoblot analysis was performed to examine the expression of MMP-9 in VCaP cells that were transfected with pCMV control or pCMV-AR vectors followed by treatment with DHT or vehicle control. b Immunoblot analysis on the expression of AR in PC-3 cells that were transfected with pCMV control or pCMV-AR vectors followed by treatment with DHT. c Immunoblot analysis on the expression of MMP-9 in PC-3 cells that were transfected with pCMV control or pCMV-AR vectors followed by treatment with DHT. d Immunoblot analysis on the expression of EGFR in PC-3 cells that were transfected with pCMV control or pCMV-AR vectors followed by treatment with DHT. Data presented is the average of at least two independent experiments (±SD). p < 0.05 is indicated by “*”, p ≤ 0.01 is indicated by “**”, p ≤ 0.001 is indicated by “***”