Fig. 1.

Implementation details of DeepCRISPR. a sgRNA encoding schema. For a DNA region, the nucleotide sequence is represented by four channels, i.e., the A-channel, C-channel, G-channel, and T-channel, and each epigenetic feature is considered as one channel. b Training details of DeepCRISPR for sgRNA on-target efficacy prediction. The Softmax and Identity functions correspond to classification and regression models, respectively. c Unsupervised deep representation learning based on billions of genome-wide sgRNA sequences. d Training details of DeepCRISPR for sgRNA off-target profile prediction. The Softmax and Identity functions correspond to classification and regression models, respectively