Fig. 5.

Automatic learning sequence and epigenetic features affecting sgRNA on-target and off-target design. a Feature saliency map for sgRNA on-target design. b Transformation of the representations of nucleotide substitution between the saliency map and traditional heatmap. Hyphens in both maps indicate that the impact of the nucleotide substitution at this position is not statistically significant by a Fisher’s exact test. c Averaged nucleotide substitution saliency map for sgRNA off-target design. Three nucleotide substitution zones are clearly identified. d Two representations for single nucleotide substitution (see the “Feature identification by deriving a class-specific feature saliency map” section