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Footprinting of site-specific DNA-binding protein. ‘F’ indicates free DNA where cells containing ptin7 plasmid have been treated with (OP)2Cu as described in Materials and Methods. ‘B’ indicates C protein bound to its site in the regulatory region of the mom operon (as shown by a bracket in the sequence below). The transcription start site is indicated with an arrow. Sequencing lanes are marked as G, A, T and C.
