Fig. 1.
Downstream insulin signaling pathway molecules display similar phosphorylation status in iPSC-derived wildtype and PSEN2N141I basal forebrain cholinergic neuroprecursors. a-e Western blot analysis of insulin signaling in iPSC-derived basal cholinergic neuroprecursor cell lines. Cells were insulin deprived overnight before the addition of corresponding insulin concentrations of 0, 10, 50, 100, 500, 1000 ng/ml. Lysates were collected after a 30-min exposure to the specified insulin concentration. Quantified western blot data was normalized to an actin standard and expressed as fold change to the 0 ng/ml vehicle control. Dose dependency was detected by 2-way ANOVA where indicated. These data correspond to results of three independent experiments. ** P < 0.01; *** P < .001. f Representative blots from a single experiment showing three of the lines