Figure 5.

Quantitative analysis of the NRG1/ErbB system expression. qRT-PCR showing the relative quantification (-ΔΔCt = log₂fold change) of ErbB receptors (a-d) and the different NRG1 isoforms (e-j). Both healthy nerves and healthy muscle fibers were analyzed and shown in the figures (green and red lines, respectively). ANKRD27 was used as housekeeping gene to normalize data. Values in the graphics are expressed as mean ± SEM. One-way ANOVA was carried out; asterisks (∗) denote statistically significant differences between autograft and hollow chitosan tube groups; hashes (#) between autograft and muscle-in-tube groups; dollars ($) between hollow chitosan tube and muscle-in-tube graft.  ^∗/#/$p ≤ 0.05, ^∗∗/##/$$p ≤ 0.01, and ^{∗∗∗/###/$$$}p ≤ 0.001. Two-way ANOVA is shown in Table S1.