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. Author manuscript; available in PMC: 2019 May 22.
Published in final edited form as: Angew Chem Int Ed Engl. 2018 Apr 26;57(21):6082–6085. doi: 10.1002/anie.201713403

Figure 3.

Figure 3

In vitro enzyme activity of EtCYP112 under an 18O2 atmosphere. (A) GC-MS chromatograms of enzyme assays with purified EtCYP112, spinach Fd and EtFdR with an excess of NADPH and GA12 or under limiting NADPH concentrations with GA12, GA15 (closed lactone) or GA24 as the substrate under an 18O2 atmosphere. (B) MS-spectra of GA9 showing the molecular ion at m/z 330 or 332 and two larger fragments corresponding to the loss of the methylated acid at C-7.