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. Author manuscript; available in PMC: 2019 May 22.
Published in final edited form as: Angew Chem Int Ed Engl. 2018 Apr 26;57(21):6082–6085. doi: 10.1002/anie.201713403

Figure 4.

Figure 4

In vitro enzyme activity of EtCYP112 with MeGA12 and MeGA15 under an 18O2 atmosphere. (A) GC-MS chromatograms of enzyme assays with purified EtCYP112, spinach Fd and EtFdR under limiting NADPH concentrations with MeGA12 or MeGA15 (closed lactone) as the substrate under an 18O2 atmosphere. (B) MS-spectra of GA9 showing the molecular ion at m/z 330 or 332 and two larger fragments corresponding to the loss of the methylated acid at C-7.