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. Author manuscript; available in PMC: 2018 Jun 27.
Published in final edited form as: Nat Protoc. 2017 Aug 31;12(9):1980–1998. doi: 10.1038/nprot.2017.083

Figure 5.

Figure 5

Optimal timing and facilitator for RV transduction to CD8+ T cells. (a,b) Wild-type P14 cells were transduced with empty-GFP RV, at the indicated time after in vitro stimulation in the presence of Polybrene (a), or on day 1 in the absence or presence of Polybrene (4 μg/ml) or RetroNectin (coated at 20 or 100 μg/ml) using six-well plates with or without tissue culture treatment (b), and analyzed for GFP expression 1 d after RV transduction. Plots were gated on live P14 cells. (a) RV transduction efficiency to P14 cells peaks at 24 h after in vitro stimulation. (b) RetroNectin showed better RV transduction efficiency as compared with Polybrene. Data are representative of two independent experiments (duplicate to triplicate per condition).