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. 2018 May 17;32:215–222. doi: 10.1016/j.ebiom.2018.05.012

Fig. 4.

Fig. 4

CD31, von Willebrand Factor and VEGFR-2 immunostaining of native, decellularized and recellularized veins.

a-c) CD31 immunostaining of cryosections. Asterisk indicates the lumen. a) Native control vein contains a confluent layer of CD31-staining cells. b) CD31-staining cell layer has been fully removed in the decellularization process. c) A CD31-positive layer has emerged on the luminal surface after recellularization.

d-f) Von Willebrand factor immunostaining of cryosections. Asterisk indicates the lumen. d) Continuous vWF staining in the native control vein. e) Continuous vWf staining of decellularized vein. f) Continuous vWF staining of recellularized vein.

g-i) VEGFR-2 factor immunostaining of cryosections. Asterisk indicates the lumen. g) Continuous VEGFR-2 staining in the cadaver control vein. h) VEGFR-2-staining layer has been removed in the decellularization process. i) No traces of VEGFR-2-positive cells in the recellularized vein.