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. 2018 May 26;32:31–42. doi: 10.1016/j.ebiom.2018.05.023

Fig. 3.

Fig. 3

P.a and E.coli activated inflammsomes in BA-MSCs of mice model.

(a) Mice were infected with P.a and E.coli via caudal vein, and then the bacterial burden in peripheral blood was analyzed. n = 4. (b) Plates cultured with E.coli and P.a. from peripheral blood. n = 3. (c) Western blot analysis of NLRP3, caspase-1, caspase-11, and IL-1β proteins in BA-MSCs from mice after bacterial challenge. n = 3. (d-f) Densitometry analyses of NLRP3, pro-IL-1β and caspase-1 p20, based on (c). mean ± SD, t-test, *P < 0.05,**P < 0.01,***P < 0.001. (g) Confocal laser scanning microscopic analysis of GSDMDC1 (green), CD51 (red) and nuclei (DAPI, blue) of bone sections from mice infected with E.coli and P.a. n = 4. (h) Western blot analysis of NLRP3, caspase-1, caspase-11, IL-1β proteins in BA-MSCs from colitis and control mice. n = 3. (i-l) Densitometry analysis of NLRP3, caspase-1, caspase-11 and IL-1β proteins based on (h). mean ± SD, t-test, *P < 0.05,**P < 0.01,***P < 0.001. (m) Confocal laser scanning microscopic analysis of GSDMD (green), CD51 (red) and nuclei (DAPI, blue) in bone sections from colitis and control mice. Each experiment represents at least four mice per group.