Fig. 4.

The impact of NLRP3^-/-BA-MSCs on IBD mice.

(a) The experimental scheme. IBD mice were transplanted with NLRP3^-/-BA-MSCs or wild type BA-MSCs, n = 5, 2 × 10⁶ cells per mouse. After transplantation for 3 days, these mice were sacrificed. (b) The clinical scores of the three groups of colitis mice. (c) The colon length of the three groups of colitis mice. (d) The H&E staining colon sections from the three groups of colitis mice. (e) These histological scores of three groups of colitis mice. (f) The location of NLRP3^-/-BA-MSCs or wild type BA-MSCs stained with CFSE in colon. (g) CFSE⁺ foci in colon sections from colitis animals treated with two types of BA-MSCs. Eight sections were observed per group. (h) Flow plots of the CFSE staining in colons from colitis mice treated with different BA-MSCs. (i) Quantity of CFSE⁺ cells according to (H). PBS: mice injected with PBS; MSC-WT: mice injected with wild type BA-MSCs; MSCs-NLRP3^-/-: mice injected with NLRP3^-/-BA-MSCs. Each experiment represents four or five mice per group. Mean ± SD, t-test, *P < 0.05,**P < 0.01,***P < 0.001.