Figure 1.

The combined CNBP repeat tract and its surrounding specific sequence with indicated primer binding sites for PCR amplification. Primers for fragment analysis are highlighted in gray and indicate the fragment analysis amplicon. Primer binding sites for sequence analysis from Radvanszky et al. (7) are indicated in italics. The combined repeat tract is denoted in bold. For statistical analysis, the specific combined repeat tract lengths were calculated by subtracting 92 bp of specific sequence surrounding the CNBP repeat (68 bp upstream and 24 bp downstream the bold repeat tract) from the measured amplicon length by fragment analysis.