Figure 5.

P4 supresses IFN-γ and IL-10 enzyme-linked immunospot (ELISpot) responses. EBV and measles responses are shown. (A) Longitudinal analysis of IFN-γ responses in P4 treated. (B) IFN-γ responses in P4 treated compared to controls. Unpaired Mann–Whitney U test. (C) Longitudinal analysis of IFN-γ responses in RU486 treated. (D) Longitudinal analysis of IL-10 responses in P4 treated. (E) IL-10 responses in P4 treated compared to controls. Unpaired Mann–Whitney U test. (F) Longitudinal analysis of IL-10 responses in RU486 treated. Gestation at sampling is indicated: in pregnant controls (●) at <20⁺⁰ weeks (N = 42), 28 weeks (N = 35), 34 weeks (N = 33); and P4 treated pregnancies (⚪) at <20⁺⁰ weeks (N = 15), 28 weeks (N = 13), 34 weeks (N = 13), 36 weeks (N = 11). For RU486 treated (N = 8) symbols represent individual patients. A dashed line represents <20 spot forming cells/10⁶. Longitudinal data were analyzed with either one-way analysis of variance with Tukey’s post hoc correction or Freidman test with Dunn’s post hoc correction depending on the data distribution. P values are two tailed and significance is defined as *P < 0.05 and **P < 0.01.