Fig. 6.
Reducing KCC2 locally in hippocampus results in neuronal loss in CA1. A. KCC2FL mice were injected with either AAV-Cre or AAV-GFP. Coronal slices prepared 21 days post-viral injection were labeled for NeuN, a neuronal marker. Region of interests of 100 μm2 were drawn in area CA1 pyramidal layer or DG granule cell layer. White boxes are magnified in B and C (Scale bar = 200 μm). B. NeuN positive cells were counted in GFP positive regions of CA1 pyramidal layer (Scale bar = 50 μm). Arrows in expanded images of NeuN labeling indicate sites of neuronal loss. There was a reduction in NeuN positive cells within the CA1 pyramidal layer in AAV-Cre mice (*p < 0.05). C. NeuN positive cells were counted in GFP-positive regions of DG granule cell layer. No significant difference in cell count was observed.