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Crosslinked CD16 enhances the IL-15–induced phosphor-STAT5 signal. Isolated NK cells (n = 9; by negative depletion) from HDs were stimulated with IL-15 (equal molar concentration to the IL-15 in TriKE [50 nM], BiKE [50 nM], BiKE + IL-15 [both 50 nM], or TriKE [50 nM]) with or without HL60 targets for 25 minutes before fixation and STAT5 phosphorylation analysis. Symbols represent distinct individuals, and statistical analysis was performed using a paired Student t test.
