Fig. 5. Molecular imaging of endogenous peroxynitrite with HKYellow in mouse livers. (a–c) Mice in the acute alcohol model group were given 50% ethanol, whereas the sham group mice received the same amount of water (n = 6 per group). (d–f) For the ischemia/reperfusion mouse model, atraumatic clips were applied to the portal vessels of mouse hepatic lobes for 1 h and then removed. The sham group mice were processed without portal vessel clamping. (a and d) Mouse livers were in situ perfused with HKYellow-AM (20 μm; perfusion rate: 2 mL min^–1, total 25 mL), cryosectioned, and mounted for fluorescence imaging. DAPI was used to stain the nucleus. (b) Quantification of the mean HKYellow fluorescence levels of liver tissue shown in (a). Statistical analyses were performed with the student's t-test. Data are shown as the mean ± S.E.M., *p < 0.05 vs. sham group. (e) Quantification of the mean HKYellow fluorescence levels of liver tissue shown in (d). Statistical analyses were performed with the student's t-test. Data are shown as the mean ± S.E.M., *p < 0.05 vs. sham group. (c and f) Mouse livers were cryosectioned and immunostained with 3-nitrotyrosine (3-NT) antibody. The scale bars represent 100 μm.