Skip to main content
. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Surg Res. 2018 May 5;229:262–270. doi: 10.1016/j.jss.2018.03.023

Figure 6. Hemorrhagic shock results in ALOX5 and ALOX5AP association and MK-886 attenuates these interactions.

Figure 6

Representative images are shown above for Control, trauma/sham shock (Trauma), and trauma with hemorrhagic shock (T/HS) tissues. In all images, nuclei are blue and tissue is gray. In the first column, ALOX5 distribution is labeled red. In the middle column, ALOX5AP is labeled green. The third column demonstrates fluorescence resonance energy transfer (FRET) signal intensity, normalized to the donor channel (FRETn/d), via a pseudo-color intensity scale with increasing intensity of the FRET signal transitioning from green (low) to yellow to red (high). In the T/HS groups (with and without DMSO), FRET signal intensity increases over both control and T/SS. MK-886 administration prior to hemorrhagic shock attenuates this effect and decreases the FRET signal intensity.