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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2017 Nov 29;339:24–33. doi: 10.1016/j.taap.2017.11.027

Figure 2. In vitro activation of NF-κB by PCB 153 is ameliorated by free radical scavenger.

Figure 2.

(A) SW480 cells were transfected with a NF-κB reporter plasmid. Cells were pretreated for 1 hr with 5mM N-Acetyl-Cysteine (NAC), a free radical scavenger, or control (PBS). Cells were subsequently exposed to LPS, PCB153, or vehicle (DMSO) for 3 hrs. (N=5) Two-way AVOVA with Tukey’s multiple comparison test. *P<0.05, **P<0.01 ****P<0.0001

(B) SW480 cells were exposed to, LPS (50ng/mL), Etoposide (10uM), PCB153 (100uM) or vehicle (DMSO) for 3 hrs. RNA was collected and RT-qPCR run for IL-6 and TNFα. (N>4) Kruskal-Wallis with Dunn's multiple comparisons test. *P<0.05, ****P<0.0001