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. Author manuscript; available in PMC: 2019 Feb 26.
Published in final edited form as: Methods Enzymol. 2018 Feb 26;601:359–389. doi: 10.1016/bs.mie.2017.11.033

Figure 3. High transfection efficiency is obtained with Cellfectin.

Figure 3

High transfection efficiency facilitates the generation of stable Kc cell lines expressing fluorescent markers of the heterochromatin domain and damage foci. To optimize this step, different transfection agents were tested following manufacturers’ instructions. Cells were transfected with 2.5 μg of a midiprep of pCopia-mCh-HP1a and the average number of cells expressing HP1a was estimated 72 h after transfection. The highest transfection efficiency obtained after several optimization steps is shown for each reagent, revealing the highest transfection efficiency using Cellfectin. Error bars show +/- SEM.