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. 2018 Jun 27;13(6):e0198362. doi: 10.1371/journal.pone.0198362

Fig 3. Octopamine is required for proboscis extension modulation by OA-VPM4.

Fig 3

(A) Double labeling of neurons in 891-Gal4 (magenta, CD8::tdTomato) with OA neurons driven by Tdc2-LexA (green, CD2::GFP). Arrows point to OA-VPM4 cell bodies in the SEZ, showing co-labeling with the Tdc2-LexA reporter. Scale = 20 μm. (B, C) Flies expressing Tβh RNAi (B) or Tdc2 RNAi (C) in 891-Gal4 neurons, were tested for PER to 10mM, 100mM, 350mM, 1M sucrose. n = 155 (B) and 120 (C) flies, mean ± SEM, Kruskal-Wallis 1-way ANOVA with Bonferroni correction. *p<0.05, **p<0.01, ***p<0.001. Pairwise comparisons to each control, asterisk colors indicate which genetic controls were compared to the experimental group.