Table 3.
Guaiac based |
FIT | miRNA | |
---|---|---|---|
Analyte | Heme | Globin | Erythrocyte-specific miRNAs |
Quantification | No | Yes | Yes |
Assay dynamic range | N/A | Higha | Higha |
Assay platform | Colorimetric | Immunochemistry | RT-qPCR |
Human-specific | No | Yes | Yes |
Dietary restriction | Yes | No | No |
Assay normalization | N/A | N/A | Yes |
Storage buffer | N/A | Protein stabilizing | Nucleic acid stabilizing |
Material costs | Low | Moderate | Moderate |
Compatibility with stool DNA test | N/A | Requires separate stool sampling and assay platform | Sampled from same buffer, same assay platform |
FIT and miRNA markers demonstrated linearity in assaying 1800–36,000 erythrocytes per µL stool homogenate (see “Results”)