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. 2018 Jun 27;4(6):eaat0062. doi: 10.1126/sciadv.aat0062

Fig. 3. Validation of the hypothesis of sequential reactions in E. coli and En. faecalis using bacteria whose genes encoding transporters and enzymes were deleted or complemented.

Fig. 3

(A and B) Extracellular putrescine concentration in cocultures of wild-type (WT) En. faecalis (Enf; SK947) and E. coli (Ec) including gene knockout mutants or complementary transformants of arginine decarboxylase (adiA) of E. coli (A) or arginine-agmatine antiporter (adiC   ) of E. coli (B). (C) Extracellular putrescine concentrations in cocultures of wild-type E. coli (LKM10096) and En. faecalis including the wild type, gene knockout mutants, or complementary transformants of agmatine-putrescine antiporter (aguD). (D) Extracellular putrescine concentration in cocultures of putrescine-deficient E. coli (SK930) and wild-type En. faecalis (SK947). Coculture of these bacteria was conducted under anaerobic conditions at 37°C for 24 hours in LB-RGC medium. (E and F) Putrescine concentrations in the content of cecal lumen (E) and colonic lumen (F) of gnotobiotic mice colonized with putrescine-deficient E. coli (SK930) and wild-type En. faecalis (SK947). Error bars represent SEs. *P < 0.05 and **P < 0.01, one-way analysis of variance (ANOVA) with Tukey’s test.