Figure 6.

Effect of SB-3CT on tPA-induced increase of caveolae-mediated transcytosis after embolic ischemia. A, Representative brain sections from the same mice described in Figure 4 were immunostained with caveolin-1 (green), CD31 (red), and Hoechst (blue) in the ischemic penumbra. 3D deconvolution was used to enhance the sharpness and contrast of fluorescent images. B, Immunofluorescent staining showed increased caveolae-1 expression in the ischemic penumbra. Caveolin-1 expression became more pronounced with tPA treatment and decreased with SB-3CT + tPA treatment. C, Magnified images from the different treatment groups. White arrows indicate areas of less caveolin-1 expression; yellow arrows indicate areas of enhanced caveolin-1 expression in the endothelial cells. Scale bars: 20 μm (A), 100 μm (B), and 25 μm (C).