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. 2018 Jun 27;5(3):ENEURO.0138-18.2018. doi: 10.1523/ENEURO.0138-18.2018

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Copyright © 2018 Taylor et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 5. — Combined ablation of NF186/AnkG reveals that AnkR fails to sustain nodal stability. A–I, PNS fibers from NF^fx;Ank^fx and SLICK-H-CreER;NF^fx;Ank^fx littermates injected at P13/14 (A–C, 5–30 dpi), P23/24 (D–F, 5–60 dpi), or P93/94 (G–I, 5–120 dpi) were triple immunostained with antibodies against AnkR (red), NF186 (blue), and NFCT (green). J–R, SCs from NF^fx;Ank^fx and SLICK-H-CreER;NF^fx;Ank^fx littermates injected at P13/14 (J–L, 5–30 dpi), P23/24 (M–O, 5-60 dpi), or P93/94 (P–R, 5–120 dpi) were triple immunostained with antibodies against AnkR (red), NF186 (blue), and Caspr (green). S, T, Graphs representing intensity quantification of AnkR in the PNS (S) and CNS (T) nodal area of SLICK-H-CreER;NF^fx;Ank^fx mice injected at P13/14 (green bars), P23/24 (blue bars), or P93/94 (red bars) normalized to age-matched NF^fx;Ank^fx control values (black bars). U, Immunoblot analysis of SC lysates from 60 dpi NF^fx;Ank^fx injected at P23/24 compared to SLICK-H-CreER;NF^fx;Ank^fx mice injected at P13/14 (30 dpi), P23/24 (60 dpi), or P93/94 (120 dpi) with antibodies against AnkR and β-actin. Arrowheads mark Ank-G negative nodes. All data are represented as mean ± SEM (n = 3–4 mice/group; 50–100 nodes per mouse; two-way ANOVA, Tukey post hoc analysis). Black asterisks indicate statistical differences between control and mutant; while colored asterisks signify differences between time points among mutants. Scale bar, 2 μm.