Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) |
Sox7 | I.M.A.G.E. clone | 40131228 | N/A |
| Gene (Mus musculus) |
Sox18 | I.M.A.G.E. clone | 3967084 | N/A |
| Strain, strain background (Mus musculus) |
Pax3GFP/+ | PMID: 15843801 DOI: 10.1038/nature03594 |
N/A | Mouse line maintained in F. Relaix lab |
| Strain, strain background (Mus musculus) |
Pax3Cre/+ | The Jackson Laboratory PMID: 15882581 DOI: 10.1016/j.ydbio.2005.02.002 |
B6;129-Pax3tm1(cre)Joe/J
MGI: J:96431 RRID:IMSR_JAX:005549 |
Mouse line obtained from J. A. Epstein |
| Strain, strain background (Mus musculus) |
Pax7CreERT2/+ (Pax7+/CE) | The Jackson Laboratory PMID: 19554048 PMCID: PMC2767162 DOI: 10.1038/nature08209 |
B6;129-Pax7tm2.1(cre/ERT2)Fan/J
MGI: J:150962 RRID:IMSR_JAX:012476 |
Mouse line obtained from C.M. Fan |
| Strain, strain background (Mus musculus) |
Tg:Pax7-nGFP | PMID: 22265406 DOI: 10.1016/j.cell.2011.11.049 |
Tg(Pax7-EGFP)#Tajb
MGI:5308730 RRID:MGI:5308742 |
Mouse line obtained from S. Tajbakhsh |
| Strain, strain background (Mus musculus) |
Sox17GFP/+ | The Jackson Laboratory PMID: 17655922 PMCID: PMC2577201 DOI: 10.1016/j.cell.2007.06.011 |
BKa.Cg-Sox17tm1Sjm
Ptprcb Thy1a/J MGI: J:123050 RRID:IMSR_JAX:007687 |
Mouse line obtained from S. J. Morrison |
| Strain, strain background (Mus musculus) |
Sox17fl/+ | The Jackson Laboratory PMID: 17655922 PMCID: PMC2577201 DOI: 10.1016/j.cell.2007.06.011 |
BKa.Cg-Sox17tm2Sjm
Ptprcb Thy1a/J MGI: J:123050 RRID:IMSR_JAX:007686 |
Mouse line obtained from S. J. Morrison |
| Cell line (Mus musculus) |
C2C12 | American Type Culture Collection (ATCC) PMID: 28966089 PMCID: PMC5640514 DOI: 10.1016/j.cub.2017.08.031 |
CRL-1772 RRID: CVCL_0188 |
Cell line maintained in E. Gomes lab |
| Antibody | anti-GFP (rabbit polyclonal) |
Life Technologies | A11122 RRID:AB_221569 |
1:500 |
| Antibody | anti-GFP (chicken polyclonal) |
Abcam | ab13970 RRID:AB_300798 |
1:500 |
| Antibody | anti-Ki67 (mouse monoclonal) |
BD Pharmingen | 556003 RRID:AB_396287 |
1:100 |
| Antibody | anti-Ki67 (rabbit polyclonal) |
Abcam | ab15580 RRID:AB_443209 |
1:100 |
| Antibody | anti-Laminin (rabbit polyclonal) |
Sigma-Aldrich | L9393 RRID:AB_477163 |
1:100 |
| Antibody | anti-Laminin (AlexaFluor647) |
Novus Biological | NB300-144AF647 | 1:200 |
| Antibody | anti-M-Cadherin (mouse monoclonal) |
nanoTools | MCAD-12G4 | 1:50 |
| Antibody | anti-MyoD1 (5.8A) (mouse monoclonal) |
DAKO | M3512 RRID:AB_2148874 |
1:50 |
| Antibody | anti-MyoD (M-318) (rabbit polyclonal) |
Santa Cruz | sc-760 RRID:AB_2148870 |
1:20 |
| Antibody | anti-Myogenin (mouse monoclonal) |
DSHB | F5D | 1:100 |
| Antibody | anti-Pax7 (mouse monoclonal) |
DSHB | PAX7-c | 1:20 |
| Antibody | anti-Pax7 (mouse monoclonal) |
Santa Cruz | sc-81648 RRID:AB_2159836 |
1:20 |
| Antibody | anti-Phospho-Histone H3 (Ser10) (rabbit polyclonal) |
Merck Millipore | 06–570 RRID:AB_310177 |
1:500 |
| Antibody | anti-Sox17 (goat polyclonal) |
R and D Systems | AF1924 RRID:AB_355060 |
1:50 |
| Antibody | Alexa 488 goat anti-mouse IgG (H + L) |
Life Technologies | A-11017; RRID:AB_143160 A-21121; RRID:AB_141514 |
1:400 |
| Antibody | Alexa 546 goat anti-mouse IgG (H + L) |
Life Technologies | A-11018 RRID:AB_2534085 |
1:400 |
| Antibody | Alexa 555 goat anti-mouse IgG (H + L) |
Life Technologies | A-21425 RRID:AB_2535846 |
1:400 |
| Antibody | Alexa 594 goat anti-mouse IgG (H + L) |
Life Technologies | A-11020. RRID:AB_141974 A-21125; RRID:AB_141593 |
1:400 |
| Antibody | Alexa 488 goat anti-rabbit IgG (H + L) |
Life Technologies | A-11070 RRID:AB_142134 |
1:400 |
| Antibody | Alexa 594 goat anti-rabbit IgG (H + L) |
Life Technologies | A-11072 RRID:AB_142057 |
1:400 |
| Antibody | Alexa 594 donkey anti-goat IgG (H + L) |
Life Technologies | A-11058 RRID:AB_142540 |
1:400 |
| Antibody | Alexa 488 goat anti-Chicken IgY (H + L) |
Life Technologies | A-11039 RRID:AB_142924 |
1:400 |
| Antibody | Cy5-goat anti-rabbit IgG (H + L) |
Jackson ImmunoResearch |
111-175-144 RRID:AB_2338013 |
1:200 |
| Antibody | Rat anti-mouse CD45-PE-Cy7 |
BD Pharmingen | 561868 RRID:AB_10893599 |
10 ng/ml |
| Antibody | Rat anti-mouse Ter119-PE-Cy7 |
BD Pharmingen | 557853 RRID:AB_396898 |
10 ng/ml |
| Antibody | Rat anti-mouse CD34-BV421 |
BD Pharmingen | 562608 RRID:AB_11154576 |
10 ng/ml |
| Antibody | Rat anti-mouse integrin-α7-A700 |
R and D Systems | FAB3518N RRID:AB_10973483 |
10 ng/ml |
| Antibody | Rat anti-mouse Sca1-FITC |
BD Pharmingen | 553335 RRID:AB_394791 |
10 ng/ml |
| Antibody | Rat anti-mouse CD31-PE |
BD Pharmingen | 553373 RRID:AB_394819 |
10 ng/ml |
| Sequence-based reagent (Pax7_foward primer) |
5’ – AGGCCTTCGAGAGG ACCCAC – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Pax7_reverse primer) |
5’ – CTGAACCAGACCTG GACGCG – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Sox7_foward primer) |
5’ – CTTCAGGGGACAA GAGTTCG – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Sox7_reverse primer) |
5’ – GGGTCTCTTCTGG GACAGTG – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Sox17_foward primer) |
5’ – GCCAAAGACGAACGC AAGCGGT – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Sox17_reverse primer) |
5’ – TCATGCGCTTCACCT GCTTG – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Sox18_foward primer) |
5’ – AACAAAATCCGGATC TGCAC – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Sox18_reverse primer) |
5’ – CGGTACTTGTAGTTGGG ATGG – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Ccnd1_foward primer) |
5’ – TTCCTCTCCTGCTA CCGCAC – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Ccnd1_reverse primer) |
5’ – GACCAGCCTCTTCCTC CACTTC – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Axin2_fowardprimer) |
5’ – AAGAGAAGCGACCCAGT CAA – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (Axin2_reverse primer) |
5’ – CTGCGATGCATCTCTC TCTG – 3’ |
Eurogentec | N/A | N/A |
| Sequence-based reagent (SoxF binding site) |
5' – CAACAATCATCATTGTTGG GGCCAACAATCTACATTGTT CAGA – 3' |
Eurogentec | N/A | N/A |
| Sequence-based reagent (SoxF binding site) |
5' – TCTGAACAATGTAGATTGT TGGCCCCAACAATGATGATT GTTG – 3' |
Eurogentec | N/A | N/A |
| Commercial assay or kit |
LIVE/DEAD Fixable Blue Dead Cell Stain Kit |
Life Technologies | L23105 | N/A |
| Commercial assay or kit |
RNasy Micro Kit | QIAGEN | 74004 | N/A |
| Commercial assay or kit |
RNeasy Fibrous Tissue Midi Kit |
QIAGEN | 75742 | N/A |
| Commercial assay or kit |
Transcriptor First Strand cDNA Synthesis Kit |
Roche-Sigma-Aldrich | 04897030001 | N/A |
| Commercial assay or kit |
LightCycler 480 SYBR Green I Master |
Roche-Sigma-Aldrich | 04887352001 | N/A |
| Commercial assay or kit |
Lipofectamine LTX PLUS reagent |
Life Technologies | 15338–100 | N/A |
| Chemical compound, drug |
Cardiotoxin | Latoxan | L8102 | 10 µM |
| Chemical compound, drug |
bFGF | Peprotech | 450–33 | 20 ng/ml |
| Chemical compound, drug |
Chicken embryo extract | MP-Biomedical | 2850145 | 0.5–1% |
| Chemical compound, drug |
Collagenase A | Roche-Sigma-Aldrich | 10103586001 | 2 μg/ml |
| Chemical compound, drug |
Collagenase type I | Sigma-Aldrich | C0130 | 0.2% |
| Chemical compound, drug |
4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) |
Life Technologies | D1306 | N/A |
| Chemical compound, drug |
Dispase II | Roche-Sigma-Aldrich | 10103586001 | 2.4 U/ml |
| Chemical compound, drug |
DNaseI | Roche-Sigma-Aldrich | 1284932 | 10 ng/mL |
| Chemical compound, drug |
Dulbecco’s modified Eagle’s medium (DMEM) |
Life Technologies | 41966 | N/A |
| Chemical compound, drug |
DMEM with GlutaMAX | Life Technologies | 61965–026 | N/A |
| Chemical compound, drug |
EdU | Thermo Fisher Scientific | C10340 | 2 μM |
| Chemical compound, drug |
Fetal bovine serum (FBS) | Life Technologies | 10270 | 20% |
| Chemical compound, drug |
Fluoromount-G | Southern Biotech | 0100–01 | N/A |
| Chemical compound, drug |
Gelatin | Sigma-Aldrich | G1890 | 0.1% |
| Chemical compound, drug |
Horse serum | Life Technologies | 26050088 | 5–10% |
| Chemical compound, drug |
Penicillin/streptomycin | Life Technologies | 15140–122 | 1X |
| Chemical compound, drug |
Tamoxifen | Sigma-Aldrich | T5648 | 5–10 µg/day |
| Software, algorithm |
Metamorph Software | Molecular Devices | RRID: SCR_002368 | N/A |
| Software, algorithm |
ImageJ | https://imagej.nih.gov/ij/ | RRID:SCR_003070 | N/A |