Table 2. List of qPCR oligonucleotides used in this study.
Pax7, Paired Box 7; Myf5, Myogenic Factor 5; Myod1, Myogenic Differentiation 1; Myog, Myogenin; Myh1, Myosin Heavy Chain 1; Ki67, Marker Of Proliferation Ki-67; Sox7, SRY-Box 7; Sox17, SRY-Box 17; Sox18, SRY-Box 18; Ccnd1, Cyclin D1; Jun, Jun Proto-Oncogene, AP-1 Transcription Factor Subunit; c-myc, MYC Proto-Oncogene, BHLH Transcription Factor; Axin2, Axin2; Ppard, Peroxisome Proliferator Activated Receptor Delta; Hprt1, Hypoxanthine Phosphoribosyltransferase 1; and TBP, TATA Box Protein.
| Antigen | Reference | Company | Ig type | Dilution |
|---|---|---|---|---|
| GFP | A11122 | Life Technologies | Rabbit IgG | 1:500 |
| GFP | ab13970 | Abcam | Chicken IgY | 1:500 |
| Ki67 | 556003 | BD Pharmingen | Mouse IgG1 | 1:100 |
| Ki67 | ab15580 | Abcam | Rabbit IgG | 1:100 |
| Laminin | L9393 | Sigma-Aldrich | Rabbit IgG | 1:100 |
| Laminin (AlexaFluor647) | NB300-144AF647 | Novus Biological | Rabbit IgG | 1:200 |
| M-Cadherin | MCAD-12G4 | nanoTools | Mouse IgG1 | 1:50 |
| MyoD1, 5.8A | M3512 | DAKO | Mouse IgG1 | 1:50 |
| MyoD, M-318 | sc-760 | Santa Cruz | Rabbit IgG | 1:20 |
| Myogenin | F5D | DSHB | Mouse IgG1 | 1:100 |
| Pax7 | PAX7-c | DSHB | Mouse IgG1 | 1:20 |
| Pax7 | sc-81648 | Santa Cruz | Mouse IgG1 | 1:20 |
| Phospho-Histone H3 (Ser10) | 06–570 | Merck Millipore | Rabbit IgG | 1:500 |
| Sox17 | AF1924 | R and D Systems | Goat IgG | 1:50 |