(A) An HIV Rev-dependent reporter cell line, Rev-CEM-GFP, was infected with an equal p24 level of HIV-1NL4-3 (Wt) or an integrase mutant, D116N. The percentage of GFP-positive cells was measured at 48 hours post infection with flow cytometry (density plot, left panel). The average mean intensity of GFP in the GFP-positive cells is also shown (histoplot, right panel). (B) The integrase inhibitor, 118-D-24 (50 µM), was used to treat Rev-CEM-GFP cells for 4 hours prior to HIV-1 infection. Cells treated with 118-D-24 or untreated were then infected with an equal p24 level of the Wt virus for 2 hours. Following infection, cells were cultured in the continuous presence of 118-D-24. The percentage (%) and average GFP intensity (M) of GFP-positive cells were measured at 48 hours with flow cytometry. All these experiments have been repeated 3 times.